Purification and partial characterization of a new variant of human prothrombin: prothrombin Metz
نویسندگان
چکیده
منابع مشابه
Purification and characterization of a variant of human prothrombin: prothrombin Segovia.
A dysprothrombin designated prothrombin Segovia was isolated from the plasma of an individual with normal prothrombin antigen and prothrombin activity lesser than 25% of the control prothrombin activity. Activation by prothrombinase complex showed a lower amidolytic than clotting activity, which suggests a lesser generation of active intermediates than normal prothrombin. When prothrombin Segov...
متن کاملPartial Purification of Human Prothrombin
Independently and almost simultaneously a number of investigators recognized the fact that at least two plasma factors are important in the conversion of prothrombin to thrombin in the presence of tissue thromboplastin and calcium. Various names have been applied to these factors and, at a recent Macy Conference on blood clotting (1), most of the investigators agreed that they could be grouped ...
متن کاملThrombin Metz: characterization of the dysfunctional thrombin derived from a variant of human prothrombin.
Thrombin Metz and normal thrombin, resulting from activation of the respective prothrombins by factor Xa in the presence of calcium, phospholipid, and factor Va, were purified by chromatography on sulfopropyl Sephadex. By physicochemical criteria, thrombin Metz is identical to normal thrombin. Its functional properties were investigated in some reactions in which thrombin is classically involve...
متن کاملProthrombin Tokushima: characterization of dysfunctional thrombin derived from a variant of human prothrombin.
A mutant prothrombin, designated prothrombin Tokushima, was purified from plasma of a proband with 12% of normal plasma clotting activity and 42% of normal prothrombin antigen. The purified preparation gave a single band with the same mobility as that of "prothrombin" by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The factor Xa-catalyzed proteolysis of prothrombin Toku...
متن کاملBlood Coagulation Induced by Iranian Saw-Scaled Viper (Echis Carinatus) Venom: Identification, Purification and Characterization of a Prothrombin Activator
Objective(s): Echis carinatus is one of the venomous snakes in Iran. The venom of Iranian Echis carinatus is a rich source of protein with various factors affecting the plasma protein and blood coagulation factor. Some of these proteins exhibit types of enzymatic activities. However, other items are proteins with no enzymatic activity. Materials and Methods: In order to study the mechanism ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1979
ISSN: 0014-5793
DOI: 10.1016/0014-5793(79)81230-2